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Where appropriate, further investigation, such as testing glutathione status or DHFR genetic variants, may help explain why some individuals respond differently to folic acid and identify when alternative forms of folate may be beneficial

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Furthermore, although its very rare, getting too much vitamin B12 can lead to toxicity, says Yuval Pinto, MD, an obesity and family medicine doctor at Johns Hopkins Medicine in Baltimore

The ATP content in the cell lysate was determined using ATP Bioluminescent cell assay kit according to the manufacturer's suggestion (Sigma, St Louis, MO) and samples were read using the TD-20/20 Luminometer (Turner Designs, Sunnyvale, CA)