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The study showed several effects on the biophysical properties of the skin: The thickness of the epidermis (the outermost layer of skin) and dermis (the layer just beneath the epidermis) was higher in smokers

(b) methodological differences such as prospective or retrospective study design, definition and classification of smoking habits (active smoker, never smokers, ex-smokers), quantification of exposure (pack years, duration of cigarette smoking) classification of CKD (proteinuria, serum creatinine levels, eGFR), duration of follow-up after cessation of smoking, presence of comorbidities, life style, health care system, and medications

Another study which used rs-fMRI also showed that smokers exhibited reduced connectivity in DMN regions and increased activity in the network related to attention after nicotine administration [21]