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Knockdown of each gene diminished GSH levels, and knockdown of Idh2 and Me1 also increased GSSG levels, thus elevating the GSSG/GSH ratio (Figure 6D)

While many of these changes have been obvious (eat more vegetables, for example), some have not with one change being questioned above all others

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in contrast, only Cu chelator treatment show potent rescue effect on such excess Cu 2+ -induced cell death, suggesting that cuproptosis is distinct from other forms of cell death