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Activity of enzyme was determined in 100 mM potassium phosphate buffer (pH 6.5) with 1 mM l-chloro-2,4-dinitrobenzene (CDNB) and 4 mM GSH as substrates by following the change in absorbance at 340 nm ( 9.6 mM 1

For a supplement to be effective, it needs to bypass the destructive environment of the stomach and the "first-pass metabolism" of the liver

Study limitations and future directions Despite the inherent amplification bias associated with the long-range PCR, which can result in variable read depth across different amplicons, our assay achieved complete coverage of all target regions and reliably detected clinically relevant variants

Ma K, Zhang L