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GTPase activity analysis To measure the GTPase activity of the DRP1 protein using an ATPase/GTPase Activity Assay kit (#MAK113, Sigma-Aldrich, USA), first, prepare a standard curve for phosphate concentration by mixing phosphate standards and purified water according to the instructions

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Similarly, the impact of renal or hepatic impairment on BPC-157 pharmacokinetics remains unexplored, though the peptide's metabolism to amino acids and apparent wide therapeutic window suggest limited risk even with reduced clearance

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