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Cells were lysed by sonication, and cell debris were removed by centrifugation at 16,300 g for 45 min at 4 C

Adena writes, It was a surreal experience watching my son march across Schoellkopf Field as he graduated from Cornell Engineering, remembering my 21-year-old self doing the same thing 31 years earlier with Jonathan seated next to methen as students on the field and now as parents in the stands. Adena and Jonathan live in Westchester County, NY, where Adena uses her law degree in projects for advocacy, civil rights, and social justice, and Jonathan is an oncologist at CareMount Medical Group

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Step 5 Remove the needle from the syringe, and dispose of it in the sharps bin