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Addition of a large quantity of the enzyme would have increased the enzymatic contribution of the total rate of reaction mixture but that would have made the rate too fast to measure accurately and conveniently, especially at high MBB concentration

At 3 DPI, biosynthetic processes of carbon-mediated metabolism, cell structure formation, amino acid metabolism and secondary metabolite biosynthesis were mostly enriched, indicating fast metabolic changes in the fungal cells (Fig

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Researchers working with BB10 peptide should calculate dosage based on the peptide amount in the vial, the volume of bacteriostatic water or sterile solvent used for reconstitution, and the desired concentration for the experiment