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Two-photon Ca 2+ -imaging and application of A and/or anticalins In vivo and in vitro two-photon Ca 2+ imaging was performed in the same custom-made multi-photon recording setup based on an upright microscope (Olympus, Shinjuku, Japan) 18

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Double immunohistochemistry of scalp tissue This assay was conducted as described previously and adapted to the antibodies used 61