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Critical points are represented by the actual relevance of the mucus layer covering the epithelium of the nasopharynx, by the interaction of the meningococcus with the different cell types of the human nasopharynx and the local microbiota, and by the mechanism of invasion of the epithelium (intracellular or paracellular)

This method is based on the color reaction between allyl thiourea and potassium ferricyanide solution in dilute acetic acid which measures the optical density of the color complex at 600 nm and it works in the range of 2.5 to 7.5 picogram of AITC per milliliter of the color solution

Even in infinite-dimensional spaces, under suitable additional hypotheses, convex functions continue to satisfy such properties and as a result, they are the most well-understood functionals in the calculus of variations