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Bornhvd et al

34 A new brain metastatic cell model, known as BR-eGFP-CMV/Luc-V5 (BRV5), based on a human-derived 435-Br1 model, which uses a brain-metastasis nude mouse inoculated with MDA-MB-435 cells, 66 was developed by passing 435-Br1 cells expressing the enhanced green fluorescent protein (eGFP) gene and Photinus luciferase (PLuc) gene through five rounds of in vivo and in vitro selection