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Downregulation or deficiency of pyruvate kinase leads to ATP depletion which in turn affects cell viability as well as accumulation of the glycolytic intermediates (2,3-diphosphoglycerate (2,3-DPG) that eventually leads to impaired glycolytic flux 46

Concerning targeted cell types, our results obtained in neonatal neural progenitor cultures and in the neonatal hypoxia model in vivo indicate that leucovorin and dyclonine act onto neural progenitor cells to promote their differentiation into oligodendroglial cells

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