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Urine must be treated with dilute hydrochloric acid (15 minutes at room temperature) to ensure conversion of acid labile hydrazones back to INH, to reflect urinary excretion reliably since these compounds are thought to be produced in the bladder

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Therefore, inhibiting GLS could effectively starve cancer cells of the glutamine essential to their survival and prevent further glutathione synthesis, thus increasing cancer cell exposure to excessive ROS levels [40]