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The columns were washed with 15 column volumes of buffer 1 followed by the elution with 200 mM imidazole, 50 mM Na x H y PO 4 , 300 mM NaCl, pH 8.0 at 4 C

10.1016/j.bbadis.2014.12.023 Summary Keywords Alzheimers disease, glutathione, oxidative stress, antioxidants, dementia Citation Braidy N, Zarka M, Jugder B-E, Welch J, Jayasena T, Chan DKY, Sachdev P and Bridge W (2019) The Precursor to Glutathione (GSH), -Glutamylcysteine (GGC), Can Ameliorate Oxidative Damage and Neuroinflammation Induced by A 40 Oligomers in Human Astrocytes

We strongly discourage any variance from the established plan