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RNA extraction and RT-qPCR Total RNA was extracted from each sucrose gradient fraction using Trizol reagent (Invitrogen)

9 (35), 821828 (2020) Niroula, A., et al.: Pigments, ascorbic acid, total polyphenols and antioxidant capacities in deetiolated barley (Hordeum vulgare) and wheat (Triticum aestivum) microgreens