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Many studies have been done to prove that hydroxocobalamin is a better form of vitamin B12, however, due to increased cost it is not used as often

In rare situations, B12 shots itching rash anaphylaxis, a life threatening condition that can cause a fast heartbeat and difficulty breathing Other side effects that can occur include: shortness of breath, fast weight gain, and swelling congestive heart failure, the inability of the heart to adequately pump blood pulmonary edema, the buildup of fluid in the lungs peripheral vascular thrombosis, the obstruction of a vein with a blood clot hypokalemia, low potassium levels that can cause irregular heartbeats and muscle weakness fever tingling, numbness, and joint pain swollen tongue polycythemia, an increase in the red blood cell mass Additionally, B12 B12 shots are not necessary for weight loss

For purification, harvested cells were first re-suspended in chilled lysis buffer (20 mM Tris [pH 7.9], 0.5 M NaCl, 5 mM imidazole, 10% glycerol, 1 mM DTT, 1 mM PMSF) and the lysis mixture was sonicated on ice

Ivm is not affiliated with Eli Lilly