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The library was screened against TNBC cells by incubating the membrane with different cells followed by labeling the bound cells with a fluorescent dye

Aging Cell 13, 631640

[0312] Additionally chimeric constructs are provided that expand beyond targeted chemotherapy to immunotherapy by incorporating, for example, providing bispecific antibodies comprising an anti-ALPP/ALPPL2 antibody attached to a second antibody that is capable of recruiting and activating immune system components or attached to a moiety that is a checkpoint inhibitor {e.g., anti- CTLA4 {e.g., comprising an ipilimumab variable region), and/or antibodies directed against PD-L1 {e.g., comprising an nivolumab, or pembrolizumab variable region), and/or antibodies directed against PD-L2

In vascular cells, 17-estradiol (E2) can upregulate the expression and activity of PGC-1 in an estrogen receptor (ER)-dependent manner ( 3.3.2 Androgens and mitochondrial dysfunction: a potential driver of ferroptosis In contrast to the protective effects of estrogen, abnormal levels of androgens (either too high or too low) are closely associated with mitochondrial dysfunction and oxidative stress