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The relationship is bidirectional and self-perpetuating: obesity-induced metabolic dysfunction leads to insulin resistance and -cell impairment, which in turn exacerbates weight management challenges

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Absorbance was calculated at an optical density (OD) of 450630 nm after an additional 2 h of CCK-8 incubation at 37C using the microplate reader (Thermo Labsystems, Finland)