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After washed with IP buffer for three times, and the binding complex proteins were eluted from the beads heated at 95 C for 10 min in 1 Laemmli sample buffer, and separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)

Earlier results suggested that concentrations lower than 50 M of SA have regulatory roles in the development of Arabidopsis roots, while higher concentrations act as stress hormones (Pasternak et al

however, a thorough investigation of methylation patterns is necessary to draw accurate conclusions 48,49

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