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RNA integrity was evaluated with a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, United States)

Oxidative stress induces cell damage by alteration of macromolecules, such as fatty acids in membrane, essential proteins, or DNA

The MIC of isoxazole 1 against H99 (32 g/mL) is near the limit of solubility (64 g/mL) and might limit our ability to apply sufficient selective pressure