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ab7291 a mouse anti-tubulin antibody (1/1000) was used to stain tubulin along with ab150120 (Alexa Fluor 594 goat anti-mouse 1/1000) shown in the top right hand panel

10.1007/112_2014_18 86 NazirogluM

While these results suggest that lysosomal cathepsins do not appear to play a role in APAP-induced hepatotoxicity, lysosomes are an important source of iron, a catalyst known to stimulate ROS generation

Elution is usually complete after 35ml of buffer is collected per 1.0ml of settled resin, provided the imidazole concentration is high enough to efficiently elute the protein of interest