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After obtaining and purifying the plasmids that were extracted using a Plasmid DNA Miniprep Kit (TsingKe, Beijing, China), the concentration and the quality of the plasmids were determined by a NanoDropND-2000c spectrophotometer (Thermo Scientific, Wilmington, USA), and then isolated bacteria were confirmed by amplification of the 16S rRNA gene

In 2013, a clinical trial comparing phentermine as monotherapy or as part of a combined therapy, took place and resulted in a weight loss of 5.1% at 28 weeks follow-up period in favor of the combined phentermine/topiramate group.(see Table 10) Table 10

NaBPC157 strongly reduced capsaicin-allodynia

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