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Anyway, since these drugs have binding affinities for 5-HT 2A in the nanomolar range, concentrations of 100 M are well above in vivo or clinically relevant levels, risking the activation of off-target receptors (for instance, 100 M NBOH is approximately 450,000 times its binding affinity to 5-HT 2A ) (Poulie et al., 2020)

Blots were then washed and incubated with appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies for 1 h, and protein bands were visualized using a chemiluminescence kit (Thermo Scientific, Rockford, IL, USA)

Epithelial heat shock proteins mediate the protective effects of Limosilactobacillus reuteri in dextran sulfate sodium-induced colitis