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Conditional knockout animals lacking C/EBP specifically in Pax7 + cells undergoing cancer cachexia showed increased apoptosis and impaired regeneration

Refmac5 54 was used for refinement and Coot 55 for model building

Raman spectra were analysed using custom MATLAB scripts for cosmic spike removal and spectral response correction (785 nm reference standard National Institute of Standards and Technology, US)

TB-500 (a Thymosin Beta-4 fragment) acts as a G-actin sequestering peptide affecting cytoskeletal remodelling and cell migration, with effects measured at the actin polymerization level rather than at receptor-pathway level