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The supernatants of the cell lysates were isolated at 12,500 g for 30 min at 4 C and were incubated with anti-Flag magnetic beads overnight at 4 C, followed by extensive washing with NP-40 Lysis Buffer

Innovative technologies that enable targeting of undruggable targets include targeted protein degradation, such as proteolysis-targeting chimera (PROTAC) molecules that can degrade a target protein by controlling the ubiquitinproteasome system

antagomir-mediated miRNA silencing is the silencing of miR-122, described in Krutzfeldt et al, Nature, 2005, 438: 685-689, which is expressly incorporated by reference herein in its entirety