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This enhanced microvascular integrity fundamentally alters the local biological environment, ensuring an exponentially higher delivery rate of oxygen, growth factors, and critical nutrients to tissues that are notoriously avascular or poorly vascularized, such as dense tendons, ligaments, and deep cartilage matrices, thereby dramatically accelerating their biological repair timelines

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Following the sgRNA library transduction at a low MOI (MOI 0.3), cells were exposed to doxycycline to induce Cas9 expression and gene mutagenesis, and then exposed to vehicle (DMSO) or palbociclib 500 nM (SF 50 ) for two weeks