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Total protein was extracted from tissues and cells using RIPA lysis buffer containing proteinase and phosphatase inhibitors

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Regular exercise can boost cortisol secretion patterns, allowing individuals to gradually adapt to pressure and thus slow down excessive cortisol responses ( 4.2.2 The impact of exercise on the HPA axis feedback mechanism Aerobic exercise and strength training can not only boost cortisol secretion patterns, but also improve the HPA axiss negative feedback mechanisms, thus facilitating normal cortisol cycling and preventing excessive activation of stress responses (Moyers and Hagger, 2023)

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