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Description

There are also specific alterations in T cell subsets associated with aging

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For IL-1 treatment, cells were incubated in plain Dulbeccos modified Eagles medium (DMEM):F12 medium containing 6 mM glucose and 2 mM glutamine overnight followed by stimulation with IL-1 (10 ng/ml) or 0.1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) (vehicle control)

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c , Western blot of HT1080 OE GFP-GPX4 WT and HT1080 OE GFP-GPX4 R152H after treatment with ferroptosis inducers with GPX4 and GAPDH antibodies, with lanes arranged for ferroptosis inducer comparison

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