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For this experiment, we grew derivatives of strain WC3110 (arsC) carrying the empty cloning vector or plasmids p PvGST35 and p PvGST41 in medium containing an initial 1 mM Na 2 HAsO 4 , and after 24 h of growth, we quantified the proportions of arsenic as As(III) and As(V) in the culture medium

Interestingly, both of these sites are known to be important for NO binding and S -glutathionylation [14, 55], and because of the nature of Cys residues, a fine-tuned combination of multiple co- or post-translational modifications of these sites (including S -palmitoylation) could be important for regulating the multiple functions of GSTP1