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A limitation of this method is that it requires the treatment of protein under anaerobic conditions, as TNB 2 readily converts to DTNB (Ellmans reagent) under aerobic conditions, leading to false positive identifications from free thiol labeling

J Am Chem Soc 113:45184523

The ESLD-associated cascade of inflammatory cytokine storms (Table 2), such as interleukin (IL)-6, IL-10, IL-8, IL-1, tumor necrosis factor alpha (TNF), fibrinogen-like protein 2, monocyte chemotactic protein 1, and interferon , promotes the occurrence of infection [10, 11]

Various treatments were applied to cells, such as PBS, CUR, CDDP, CUR + CDDP, and CDCZA in acidified DMEM (pH 6.5) for 4 h