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During the -oxidation of palmitic acid (C16:0), through degradation of two carbon fragments, total ATP and net ATP generated is: Carbon-carbon cleavage 7 cleavage (shown by red lines) = 5 ATP per each cleavage (1 FADH + 1 NADH) Oxidation of acetyl CoA 8 acetyl CoA units entering the TCA cycle = 12 ATP per acetyl CoA unit Total ATP for 1 mole of palmitic acid (C16:0) = 35 ATP + 96 ATP = 131 ATP = 7 cleavage points (7 5 = 35 ATP) + 8 acetyl CoA units (8 12 = 96 ATP) Net ATP generated by 1 mole of palmitic acid (C16:0) = 35 + 96 2 = 129 ATP (Two ATP used for initial activation and are then subtracted from 131 ATP) Fatty acids produce higher levels of ATP than carbohydrates because they are less oxidised