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Treatments were administered with or without 300 ng/ml Doxycycline, 5 M A, 350 M BSO, 5 M DMF, and 0.1 M Fer-1, as indicated in the culture medium

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The serum samples were then stored at -20 C for future experimental use

In addition, the protein adductions of reactive metabolites of furans were determinated as Cys-and Lys-based protein adductions with the reactive metabolites (Wang et al., 2015)