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A nutrient-dense diet supplying methylation cofactors (folate, betaine, vitamin B12, vitamin B2, etc.) is foundational

Plasmid DNA construction Full-length coding sequences encoding GSTM2 (NM_001142368.1) and PCYT1A (NM_005017.3) were amplified using a cDNA library of HepG2 cells and then subcloned into the pcDNA3.1 vector (gifted from Prof