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Complete protocols by life stage 30s woman protocol (prevention + optimization) Goal : Maintain youthful vitality, prevent early aging Core protocol: Ipamorelin: 100mcg 2x daily CJC-1295: 1mg 2x weekly Optional additions: NAD+ peptides if energy is concern BPC-157 if active/injury-prone GHK-Cu topical for skin prevention Duration : 12-16 weeks, assess results, continue or cycle Cost : $250-400 monthly (core), $400-600 with additions 40s woman protocol (hormone support + anti-aging) Goal : Combat hormonal decline, maintain metabolism and vitality Core protocol: Ipamorelin: 150-200mcg 2x daily CJC-1295: 2mg 2x weekly GHK-Cu: Topical serum to face 2x daily Optional additions: NAD+ peptides for energy Semaglutide if weight gain occurring Duration : Long-term (years), this is maintenance for 40s Cost : $400-600 monthly Menopause protocol (symptom management + health) Goal : Manage menopause symptoms, prevent rapid decline Core protocol: Ipamorelin: 200mcg 2x daily CJC-1295: 2mg 2x weekly PT-141: 1-2mg as needed for libido (1-3x weekly) For weight gain: Add semaglutide (titrate to 1-2mg weekly) For severe symptoms: Add NAD+ peptides for energy Add GHK-Cu for skin/inflammation Duration : Throughout menopause transition (2-5 years), then transition to post-menopause protocol Cost : $500-800 monthly Post-menopause protocol (longevity + vitality) Goal : Healthy aging, prevent age-related decline Core protocol: Ipamorelin: 100-200mcg 2x daily CJC-1295: 2mg 2x weekly NAD+ peptides: 10mg 2x weekly BPC-157: 250mcg daily (maintenance) Duration : Lifelong for optimal healthspan Cost : $500-700 monthly ROI : Preventing one major health issue (fracture, cognitive decline, metabolic disease) pays for decades of peptides

6df and Supplementary Fig

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Finally, we also compute the variance explained in the dependent clinical set by the biomarker set