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For single IF staining, cells were incubated with Alexa Fluor 647-conjugated anti-AR antibody for 1 h at RT or with anti-BCL-2 antibody (supplementary Table 2) for 4 h at RT, followed by appropriate Alexa Fluor-conjugated secondary antibody incubation

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In addition, individual compounds of the herbal extracts are barely studied even in experimental animal models

Treatment with N-acetylcysteine may be used up to 24 hours after ingestion of Napa Extend