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Finally, we analyzed the efficiency of In vivo gene editing by delivering Cas9 mRNA and gRNA complexed with our lipids, and observed over 40% knockout of transthyretin (TTR) gene after a single injection

Then, Western blotting (WB) analysis was employed to quantify the phosphorylation levels of P65 within the NF-B signaling pathway, along with those of ERK and P38 in the MAPK signaling pathway