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Mitochondrial-derived ROS and mtDNA may activate signaling pathways like the NLRP3 inflammasome, which promotes the release of pro-inflammatory cytokines, including IL-1, IL-6, and TNF-

Centrifugation of the supernatant was then performed (12,000 g , 3 min) to obtain the mitochondrial fraction, which was washed in buffer C (320 mM sucrose