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Detection of deposited TCC (C5bC9) was done using a biotinylated mouse monoclonal antibody specific for a C9 neoepitope exposed upon C5b binding (Diatec Monoclonals, DIA 011-01) (diluted 1:5000 in PBS/T/S) together with AP-conjugated streptavidin (diluted 1:5000 in PBS/T/S) (Cytiva Life Sciences) and visualized by addition of AP substrate (Sigma-Aldrich)

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The predisposition of different variants enhancing the susceptibility of oxidizing denatured Hb (Heinz bodies) to metabolites other than bilirubin is an area that needs to be explored to gain new insights on the heterogeneity of this disease phenotype

La vitamine C, quant elle, stimule la production de collagne, ce qui attnue les ridules