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Weekly injections could work, but we need to explore simpler options, like a food additive or ointment, said Le Poole

Biochemistry of B12-cofactors in human metabolism

the membrane potential was held at -70 mV at room temperature (25C) during a period of 0.5-2 h after removal of the culture from the incubator

For immunostaining analysis of apoptotic GAD67-positive cells, the In Situ Cell Death Detection Kit, Fluorescein (Roche, #11684795910) was used along with a polyclonal rabbit antibody to GAD67 (Abcam, #ab97739, 1:100) and anti-rabbit IgG Alexa Fluor 555 conjugated antibody (Invitrogen, # A31572, 1:400)