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The supernatants were used for enzyme and protein analysis

The AMPK pathway is triggered during an energy crisis (e.g., low ATP levels, high AMP levels or nutrient deprivation), inhibiting mTOR activity and inducing catabolic processes such as autophagy

Accessed 12/10/2022b, CDC

Some of the most common side effects of iron deficiency include: shortness of breath (people often report that they cannot get enough air into their lungs) mood swings or irritability extreme fatigue (people commonly report sleeping for 8 + hours and still waking up exhausted) chronic recurrent infections (cold and flu) craving ice hair loss muscle pain exercise intolerance headaches weakened or spooned nails (see the picture on the right) restless legs syndrome angular stomatitis (cracking in the corner of the lips) chapped lips easy bruising tachycardia sensitivity to bright lights **Children with iron deficiency often exhibit lethargic behavior, poor growth, and may experience unexplainable fever spikes as well as chronic recurrent infections