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The half-life of tirzepatide is about 5 days, which means steady state arrives around week 3 to 4 at each dose

The thermal cycling conditions consisted of the following: cDNA synthesis for 10 minutes at 50 C, initial denaturation and Taq Polymerase activation for 5 minutes at 95 C, followed by 40 cycles of denaturation at 95 C for 10 seconds and annealing/extension at 60 C for 30 seconds

apical to basolateral (A B) and basolateral to apical (B A) permeability compounds were added at 10 M concentration to the apical (A) side and basolateral (B) side, respectively, and the corresponding amount of permeation was determined by measuring compound concentration on the B or A side