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doi: 10.1210/endo-61-6-713

Membranes were blocked with Tris-buffered saline (TBS) and 0.1% Tween-20 (TBS/T) containing 5% bovine serum albumin (Sangon Biotech Co., Ltd., Shanghai, China) at 37C for 2 h, and incubated overnight at 4C with primary antibodies (Abcam, Cambridge, MA, USA