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Other studies have instead observed how important the role of socio-economic status and unhealthy life style, which includes smoking, poor diet, poor exercise and obesity [10, 11]

Then, cDNA was prepared using high-capacity RNA- to-cDNA master mix (Applied Biosystems, 4368814) according to kit instructions

Epigenetic effects

Exendin-4 (the glucagon-like peptide 1 receptor agonist) significantly increases SIRT1 expression, decreasing Txnip expression and XBP1s nuclear translocation through suppressing the acetylation level of H3K9 in the Txnip promoter region mediated by SIRT1, ultimately improving the renal pathological injury induced by HFD/HG