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Supernatant was discarded, and the cell pellets were washed with wash buffer (10 mM Tris-HCl, pH = 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA with protease inhibitors) and resuspended in the sonication buffer (10 mM Tris-HCl, pH = 8.0, 1 mM EDTA, 0.1% SDS with protease inhibitors)

Atractylodin, the principal bioactive compound in Atractylodes macrocephala , possesses a range of pharmacological properties, including anti-inflammatory, antitumor, cardioprotective, and antioxidant activities (82)

Transcriptome modulation following administration of luteolin to bleomycin-etoposide-cisplatin chemotherapy on rat LC540 tumor Leydig cells

Folate-deficiencyinduced homocysteinaemia in rats: Disruption of S-adenosylmethionine's co-ordinate regulation of homocysteine metabolism