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Garrett JE, Garrett JE, Metzger E, et al

An important tool to assess the pathophysiological relevance of a mode of cell death or a specific mechanism such as LPO is to use a positive control, e.g., APAP + Fe 2+ or APAP in vitamin E-deficient mice

Assay buffer B contained 25 mmol/L KH 2 PO 4 , 25 mmol/L K 2 HPO 4 , (pH 7.2), 2.5 mmol/L rotenone in ethanol, 2 mmol/L KCN, 0.01 mg/mL antimycin A, 50 mol/L DCPIP