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After 24 h, half the supernatant was removed and replaced with 100 l of fresh medium containing OligoG (8%) and nystatin (4 g/ml) (final concentration of 4% OligoG and 2 g/ml nystatin) with a further 24 h incubation (20 rpm rocking, 37C

Each batch undergoes comprehensive testing for heavy metals, microbial contamination, and endotoxins through third-party laboratories including SGS, providing documentation that supports regulatory submissions in markets including North America, Europe, Switzerland, South Africa, Southeast Asia, and Australia

They determined regulation of fatty acid metabolism enzymes and cholesterol metabolism enzymes in diabetic kidney