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PMID: 1562181, DOI: 10.1146/annurev.ph.54.030192.002215

Using confocal microscopy, we evaluated the change of fluorescence level in neurites relative to the cell body after 15, 30 min, 1, 2 and 4 h of incubation with 100 nM A 42 (Figures 1C,D)

H & E staining was performed to evaluate tissue architecture while Periodic Acid Schiff (PAS) reagent was used to stain for glycogen according to well-established protocols