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The rate-limiting enzyme in this pathway is Indoleamine 2,3-dioxygenase 1 (IDO-1), which is upregulated by pro-inflammatory agents such as interferon- (IFN-) and tumor necrosis factor-alpha (TNF-), interleukin-1 (IL-1), interleukin-12 (IL-12), all cytokines that are released during inflammatory responses (Yang et al., 2024)