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The BD MICRO-FINE+ 0.5 mL is widely available with reliable IU calibration

In this study, the difference in the ratio of Col1:3 between the groups was more significant than comparing Col-1 or Col-3 alone

After overnight incubation, the medium was changed to 90% of Williams E Medium and supplemented with 12.5% HepaRG preinduction and toxicity medium supplement (Lonza, catalog: MHPIT) and 25 U/mL penicillin-25 g/mL streptomycin, followed by 72 h incubation