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A key factor in the experimental protocol is the use of Matrigel as an ECM scaffold, because of its heterogeneity and its poorly defined composition

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In the mitochondrial matrix, moderate elevations in Ca2+ levels activate key dehydrogenases of the TCA cycle, such as isocitrate dehydrogenase (IDH) and -KG dehydrogenase (-KGDH), thereby enhancing NADH production, fueling the ETC, and increasing ATP synthase activity (88)