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S2CID 4421515

This work was supported by the National Recovery and Resilience Plan (NRRP), Mission 4 Component 2 Investment 1.4 - Call for tender No

UCHL3 was diluted in reaction buffer (25 mM Tris, 50 mM NaCl, 5 mM DTT, 0.1% (w/v) BSA, pH 7.4) to 62.5 pM, yielding a final well concentration of 25 pM